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agarose gel has melten

Started by Pat, Jun 09 2011 12:19 AM

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4 replies to this topic

#1 Pat

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Posted 09 June 2011 - 12:19 AM

Dear all:
there's someone in my lab had this problem that her
gel melted during the electropherosis, does anyone know what might've happened?

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#2 GNANA

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Posted 09 June 2011 - 12:33 AM

The problem might be with the Running buffer...What conc of TAE used for running ?, if used too concenterated would get heated up while running and that might have caused the gel melting...

Gnana...

I would prefer being perfectionist rather than a passionist in Research.

I always had an alternate hypothesis....

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#3 Adrian K

Legendary Graduate Beggar

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Posted 09 June 2011 - 12:34 AM

You do not change your running buffer for quite some time... accumulated too much salt > high voltage > too hot > melt....
or... too high running voltage.

Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.

..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...

"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong

"It's all just DNA. Do it."---phage434