i wonder if i would like to get pure gDNA without RNA contamination, in which step should i add RNase?
The protocol that i'm using:
--> lysozyme treatment overnight
--> add SDS/proteinase k + RNase and incubate at 370C for 30 min
--> incubate at 560C for 3 hours
--> phenol/chloroform extraction x 3
--> chloroform extraction
--> sodium acetate + isopropanol
i'm still getting some rRNA contamination when view under gel.
Wonder what's wrong in my extraction steps.
Anybody could give any advice?
Edited by 123blockhead, 08 June 2011 - 07:28 PM.