I have to use pH sensitive fluorescent probe for determining the intracellular pH of my cells in experimental conditions. I decided to use SNARF-4 AM ester. The procedure seems relatively simple using a fluorescence plate reader. However, I am having a hard time finding a good protocol for doing the calibration. Literature has several different method and I have chosen Nigericin calibration with high K+ buffers. However I can't seem to find the recipe for making the buffer. Anyone has any experience using those or can help me figure out? Help is appreciated.
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Anyone have experience using SNARF (pH sensitive fluorescent probes)
1 reply to this topic
Posted 15 January 2012 - 01:32 AM
Hi pm1234! i have also decided to use SNARF . But i also cannot find the protocol for making all the buffers. Have you finally made one? If you had , could u kindly share it with me please?THX!!