MSC in culture - stop growing?
Posted 07 June 2011 - 08:14 AM
This has happened at various passage number, it has happened at pass 5, 10, and recently transduced cells (GFP+) at pass 23 stopped expanding.
Does anyone have experience with this? What could be causing it, and can I kick start them again?
Posted 05 July 2011 - 06:36 AM
Edited by bioking, 05 July 2011 - 06:36 AM.
Posted 16 August 2011 - 03:36 PM
If you make MSC culture confluent, they often stop growing even passage 5.
so it might be the reason??