2 replies to this topic

[ziggyzabel]

I have rat mesenchymal stem cells derived from bone marrow in culture. They expand very well, but lately they have randomly stopped expanding. I am using the same culture media and split ratio as always, but sometimes after a split the cells just don't grow. They are all alive, and morphology looks correct, but they can sit in culture for greater than 2 weeks and never divide.
This has happened at various passage number, it has happened at pass 5, 10, and recently transduced cells (GFP+) at pass 23 stopped expanding.
Does anyone have experience with this? What could be causing it, and can I kick start them again?
Thanks!

[bioking]

what culture are you using for the MSC anyways? what did you do to isolate the MSC?

Edited by bioking, 05 July 2011 - 06:36 AM.

[Rnotk]

MSC is somewhat sensitive to contact inhibition.
If you make MSC culture confluent, they often stop growing even passage 5.
so it might be the reason??