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[biorockstar]

Hi everyone,

I am trying to inhibit DNA-PK (DNA-dependant protein kinase), in vitro, in hybridoma cells. I have researched and, so far, wortmannin seems like the most common inhibitor to use. I have also, however, found some information on vanillin as well.

Does anyone have any experience working with either of these chemical inhibitors? If so, can you share your experience (pros/cons, what worked, what did not)?

Thanks in advance!

[castleinthesky]

DNA-PK can cooperates with ATR and ATM to phosphorylate proteins involved in the DNA damage checkpoint. PI-103 is also considered as a potent and selective DNA-PK inhibitor.