DNase treatment after RNA extraction using RNeasy kit of qiagen
Posted 07 June 2011 - 02:58 AM
I isolated plant leaf RNA using RNeasy kit of qiagen. Kit itself assure DNA contamination free RNA but some minut quantity I recorded on my agarose gel. I've not done on-column DNase treatment but now I want to treat my samples with DNase. Can I use NEB DNase for this RNA. second RNA cleanup procedure is required after this treatment or not? How much RNA I should treat with DNase?
Posted 19 June 2011 - 08:56 PM
Posted 21 June 2011 - 03:15 AM
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