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THP-1 cell line


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3 replies to this topic

#1 akbar

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Posted 07 June 2011 - 12:18 AM

I had thawed a new vial of THP-1 cells which I had frozen in 2007 but they are not growing now. what can be the problem.

#2 eck

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Posted 12 June 2011 - 07:43 AM

What's your media? What does it contain?

I use: RPMI + 10% FCS + 1% P/S + 1% glutamax + .1% B-mercaptoethanol

They tend to double in 36 hours or so.
What's your splitting density?

#3 zienpiggie

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Posted 12 June 2011 - 08:12 AM

Was it frozen in -80 or in liquid nitrogen? And did you have accidents of liquid nitrogen loss at any point?

#4 Anula

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Posted 03 October 2011 - 12:49 AM

I treat my THP-1 with PMA. I prepared 50 ml medium RPMI with 10% inactivated serum, antibiotics solution and 50 ng/ml PMA. I would like to know If I can store this medium in refrigerator for a few days? MAybe I should do a fresh medium for every experiment….




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