Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

ELISA plate coating with plasma membrane vesicles

  • Please log in to reply
1 reply to this topic

#1 lovenergy



  • Active Members
  • Pip
  • 7 posts

Posted 06 June 2011 - 05:46 PM

Has anyone coated their ELISA plates with plasma membrane vesicles? Any suggestions on how this might be done? Thanks!

#2 Ben Lomond

Ben Lomond


  • Active Members
  • PipPipPipPipPip
  • 75 posts

Posted 08 June 2011 - 04:12 PM

A quick literature search shows this has been done mainly back in the 80 and 90s in a number of applications. The approach was to use a regular coating approach, either neutral PBS or basic carbonate buffers and low to mid microgram per mL concentration of total protein using maxisorp, polysorp or other vendors' equivalents.
Fixation with low concentration glutaraldehyde (0.5% or less in PBS) may make the vesicles more stable if the antigen still remains accessible, but this might make blocking more challenging. Active coating plates (see Pierce) may also be helpful.

Start simple before adding potentially unhelpful complexity.

Good Luck!

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.