I read that Taq should not be freezed. The commercial stock solution contains 50% glycerol so it does not freeze at -20. However a lot of suppliers sell 2X master mixes with Taq pre-added. These master mixes obviously do not contain 50% glycerol because that's inhibitory to PCR. So perhaps they freeze at -20. The question is, can I prepare my own master mix with Taq added to the mastermix and store at -20? Is it ok if Taq freezes within the master mix?
Also, what if I need to store my PCR materials for a long term? Can I store the stock solutions of dNTPs, Primers and Taq at -80 C without problems? Has anyone tried that already?
Thanks a lot.
Edited by Bassaml7, 06 June 2011 - 08:00 AM.