I'm using qPCR to assess the amount fo viral RNA in different samples. One sample originally had a Ct value of 22.83; after treatment of the sample it's Ct value decreased to 25.95. The efficiency of the PCR is 100%. I would say that there is 8.69 (i.e. 23.12) times more RNA in the sample before treament, but my supervisor describes the change as a 90% loss from original sample. How is this calculated?! Maths most definitely not being my strong point! Thanks :-)
Edited by katcvet, 06 June 2011 - 12:53 AM.