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[yimao]

hi, guys, i was doing lysis of cultured mamallian cell for 2DE studay, and i used Lysis Solution with the composition of:

8M urea, 4% w/v Triton X-100 (a mild detergent)
then votex for 5 min

my Q is:
will this method break organelles like: necleus, microbodies, mitochondrial and golgi apparatus?
or am i just get to the cytosol only?

thx~