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will this method break organelles?

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#1 yimao



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Posted 04 June 2011 - 07:52 PM

hi, guys, i was doing lysis of cultured mamallian cell for 2DE studay, and i used Lysis Solution with the composition of:

8M urea, 4% w/v Triton X-100 (a mild detergent)
then votex for 5 min

my Q is:
will this method break organelles like: necleus, microbodies, mitochondrial and golgi apparatus?
or am i just get to the cytosol only?


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