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Functions of restriction buffer components


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#1 Ikar

Ikar

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Posted 04 June 2011 - 01:04 PM

Hi,

let's assume I have a EcoRI buffer with this components:

1X Composition
50 mM Tris-HCl (pH 7.5 at 37C)
10 mM MgCl2
100 mM NaCl
0.02 % Triton X-100
0.1 mg/ml BSA

Does anyone now the exact function of these components?

My assumptions
- Tris-HCl: simple buffer fo optimal pH conditions
- MgCl2, co-factor of EcoRI?
- NaCl ?
- Triton?
- BSA: enhances protein stability and binds to contaminants; binds to reaction tube surface and prevents enzymes to bind there




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