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Nisin concentration


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#1 Biochemilab

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Posted 04 June 2011 - 12:50 PM

Hallo dear members,

I would like to know some methods to measure the exact concentration of my protein ( Nisin). Can I find some help from you.
Thanks in advance,

#2 mdfenko

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Posted 06 June 2011 - 07:33 AM

purified or as part of a mixture?

if purified then you can perform one or two of any number of protein assays, depending on concentration.

one of the more exact assays is based on the kjeldahl (or micro-kjeldahl) nitrogen determination.

if part of a mixture then you can determine the concentration of the mixture then determine the percentage of the mixture that is your protein (by page or gel filtration).

Edited by mdfenko, 06 June 2011 - 07:33 AM.

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