Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Nisin concentration

  • Please log in to reply
1 reply to this topic

#1 Biochemilab



  • Members
  • Pip
  • 1 posts

Posted 04 June 2011 - 12:50 PM

Hallo dear members,

I would like to know some methods to measure the exact concentration of my protein ( Nisin). Can I find some help from you.
Thanks in advance,

#2 mdfenko


    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,202 posts

Posted 06 June 2011 - 07:33 AM

purified or as part of a mixture?

if purified then you can perform one or two of any number of protein assays, depending on concentration.

one of the more exact assays is based on the kjeldahl (or micro-kjeldahl) nitrogen determination.

if part of a mixture then you can determine the concentration of the mixture then determine the percentage of the mixture that is your protein (by page or gel filtration).

Edited by mdfenko, 06 June 2011 - 07:33 AM.

talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.