Hepes Tris pH 6 buffer
Posted 03 June 2011 - 03:11 AM
I need to prepare a buffer which has been described in several papers as "20mM Hepes/Tris (pH Hepes/Tris 6.0)"
I'm not sure what the 20mM refers to (is it the Hepes or Tris) and how to get it to pH 6.0
Any help would be much appreciated.
Posted 03 June 2011 - 05:16 AM
Roughly speaking, make 40 mM solutons of Tris base and HEPES (may not go into solution) and titrate to pH 6.0. Given the solubility of HEPES, you might want to start with solid HEPES in the correct amount and add 40 mM Tris-base until it is dissolved, then titrate to pH 6.0.
Posted 03 June 2011 - 06:04 AM
I'll give it a go.