i am encountering this bizzare problem with my PCR reactions.... the amplified product seems to have suddenly dissappeared.. i was getting pretty good results until a few days back. i have tried with fresh components also.
can someone please help...
thanks......
0
2 replies to this topic
#2
Adrian K
-
- Global Moderators
-
- 684 posts
Legendary Graduate Beggar
24
Excellent
Excellent
Posted 02 June 2011 - 11:48 AM
is your PCR machine in order: calibrated blot?
New DNA template? Degraded template?
New DNA template? Degraded template?
Expecting the world to treat you fairly because you are a good person is like expecting the lion not to attack you because you are a vegetarian.
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
..."best of our knowledge, as far as we know this had never been reported before, though I can't possible read all the published journals on earth, but by perform thorough search in google, the keywords did not match any documents"...
"what doesn't kill you, makes you stronger"---Goddess Casandra reminds me to be strong
"It's all just DNA. Do it."---phage434
#3
evd
-
- Members
-
- 2 posts
member
0
Neutral
Neutral
Posted 03 June 2011 - 08:59 AM
the machine is fine.... it was giving amplification until recently.
the template is the same as the one being used previously. so i am totally confused and have no clue as to what is happening....
the template is the same as the one being used previously. so i am totally confused and have no clue as to what is happening....
