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issues in PCR amplification


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#1 evd

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Posted 02 June 2011 - 09:06 AM

i am encountering this bizzare problem with my PCR reactions.... the amplified product seems to have suddenly dissappeared.. i was getting pretty good results until a few days back. i have tried with fresh components also.
can someone please help...
thanks......

#2 Adrian K

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Posted 02 June 2011 - 11:48 AM

is your PCR machine in order: calibrated blot?
New DNA template? Degraded template?
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#3 evd

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Posted 03 June 2011 - 08:59 AM

the machine is fine.... it was giving amplification until recently.
the template is the same as the one being used previously. so i am totally confused and have no clue as to what is happening....




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