Transfection of CHO cells with Fc receptor
Posted 01 June 2011 - 11:44 AM
Posted 02 June 2011 - 02:06 AM
I too came across a similar situation, in which i could express a contruct in 293 or Hela but couldnt make it express in some of my cancer model cell lines which is definetely not transfection resistant (though not highly competent like 293), as i could express some other plasmids with ease. the only difference i could say between these cell lines is that my cancer models has endogenous expression whereas the 293 or hela doesnt...so my concern is would the cells wont allow to express the exogenous if it already has the same protein highly expressed endogenously???...if anybody came across such a phenomenon and if you have any explanation for this please shed your inputs...thanks in advance...bye..
Edited by GNANA, 02 June 2011 - 02:09 AM.
I always had an alternate hypothesis....