Problems with PCR in general
Posted 01 June 2011 - 10:04 AM
Posted 02 June 2011 - 02:06 AM
I'm not getting the concentration question? You need to dilute your DNA and don't know how? What is DNA concentration of your sample?
If your band is faint, you need to optimise the reaction, not only template amount, but annealing temperature, primer concentration, Mg2+ concentration, number of cycles (starting with 30). You didn't write any details about your PCR reaction, so it's hard to tell.
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