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EMT induction with TGFb


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#1 marco86

marco86

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Posted 01 June 2011 - 09:10 AM

Hello,

is there somebody working on epithelial to mesenchymal transition (EMT)to help me?

I am trying to induce EMT with TGFb1 on MDCK cells but the phenotype I get is not strong,


I know that crytical elements to trigger EMT are:


-Serum starvation

-Cell confluence at the time of stimulation


Is there somebody who could advise me?

Thanks a lot

Marco




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