IC50 calculation software (need help)
Posted 01 June 2011 - 08:34 AM
Posted 02 June 2011 - 02:34 PM
it is easy to use
choose the xy table+ graph
them use your x valeus log (10)
analise with the non linear fit curve log (agonist)vs response
hope that helps
also see the topic http://www.protocol-...w-to-calc-ic50/ they have good sugestions
Edited by martha2, 02 June 2011 - 03:01 PM.
Posted 02 June 2011 - 02:41 PM
MasterPlex ReaderFit 2010
i never used that program but it seems easy to use
you can see the video
Posted 01 November 2011 - 12:42 AM
Posted 08 January 2012 - 07:29 AM
Posted 25 August 2012 - 10:16 AM
You should never use Excel for IC50 estimation. Your viability data is not linear or fits simple log transformation. For accurate calculation of IC50, a nonlinear curve fitting program that uses the Four Parameter Model is needed.
A paid program such as GraphPad Prims is great, but there is a free online program BioDataFit, which produces the same accuracy as GraphPad and it is easier to use.
To learn how to prepare the raw data for inputting into an IC50 program, read here. To learn what to do if your experimental data does not fit the Four Parameter Model, which can be very frustrating to new users, read here.
Good luck and hope you all will smoothly get your cytotoxicity experiments done and get publication quality IC50s from these tips.
Edited by AquaPlasmid, 25 August 2012 - 10:18 AM.
Posted 27 August 2012 - 02:13 PM
You should never use Excel for IC50 estimation.
This should read “should never use Excel for IC50 estimation if you are unsure of what you are doing”. I haven’t come across anything in Graphpad that can’t be done in Excel or libreoffice. Graphpad is just a spreadsheet with less flexibility.
Posted 27 August 2012 - 04:05 PM
DRT, we would be happy to try your Excel IC50 macro if you would post it up, and compare it to BioDataFit, in case BioDataFit goes offline.