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protein level expression


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#1 silvepa23

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Posted 31 May 2011 - 09:20 PM

Hi,



thanks in advance for any help. I wanted to know how to estimate the expression level of a protein that has been expressed in e coli. how do i go about doing it and what are the steps? thanks again.

#2 silkworm

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Posted 06 June 2011 - 07:29 AM

You can measure your protein level with ELISA kit, Western Blot with antibodies, SDS-PAGE with a titration of loading controls ( like 0.1, 0.2, 0.5, 1.0 ug BSA per lane ). Please refer to Novateinbio.com for ELISA protocol and antibodies.




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