1 reply to this topic

[silvepa23]

hi,

I was hoping someone couple help me.  I have posted 2 links for two vectors.

http://www.genecopoe...eceiver-B03.pdf

http://www.genecopoe...eceiver-B03.pdf

I was wondering what are the TEV and EK sites?  Are they where the tag can be leaved?  

I was also wondering what the MC1 and MC2 was?



Thanks a lot for all your help.

[protolder]

silvepa23, on 31 May 2011 - 09:04 PM, said:

hi,

I was hoping someone couple help me.  I have posted 2 links for two vectors.

http://www.genecopoe...eceiver-B03.pdf

http://www.genecopoe...eceiver-B03.pdf

I was wondering what are the TEV and EK sites?  Are they where the tag can be leaved?  

I was also wondering what the MC1 and MC2 was?



Thanks a lot for all your help.

Hola, both sites TEV and EK sites are recognition sites of proteases one from a tobacco virus and enterokinase and they are designed to give the untagged protein after purification and digestion. TEV protease add a Nt Ser to your protein and nothing for enterokinase. TEV site Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser. and Ek site Asp-Asp-Asp-Asp-Lys/. MC1,2 are sites for cloning with restriction enzymes.You have to have all the sequence of both vectors and if the cloning sites aren´t describe introduce the sequences in any REpg. as NENcutter.(by the way you have send only the map of one of the plasmids). Buena suerte