1 reply to this topic

[myparvo]

Hi,

I would like to know is anyone could provide the information about doing the biotinylation of each single protein in cell lysate.

Thanks.

[BioMiha]

I'm not sure how you could biotinylate each single protein in a cell lysate. There are comercial biotinylation reagents available to add the biotin tag to all proteins that have primary amines available. I have had reliable results with the Pierce biotin reagent sulfo NHS biotin.