Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

problem of 2-DE with flower tissue


  • Please log in to reply
No replies to this topic

#1 mimosa

mimosa

    member

  • Active Members
  • Pip
  • 23 posts
0
Neutral

Posted 31 May 2011 - 07:52 AM

i extracted protein from 0.2g flower sample by phenol extraction.
the protein content in the sample was about 1500ug/ml.
then about 100ug protein was loaded in IPG strip.
the focusing time needed up to 7 hours.
unfortunately, no spots were found in the sds-page.
is that the problem of my phenol extraction?
what the idle time for focusing step for 7cm IPG strip?




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.