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Serum storage


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#1 Fanny

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Posted 31 May 2011 - 01:26 AM

Hello,
I have serum of patients that I want to analyse later by ELISA.
They were first stored at -80蚓. Then I thawed them and aliquot them in small tubes that I store at -20蚓.
For each assay I will only use a very small quantity (1 or 2無) and my aliquots are 30無 so I don't want to use a new aliquot each time because I would waste a lot. So my question is how many time can I thaw and freeze again these aliquots without affecting the serum composition?
Thanks for your help.

#2 PAO_ahac

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Posted 09 June 2011 - 02:58 AM

the composition remains the same however you may degrade your protein of interest. Best suggestion I can give is to do an experiment with one sample and run it through several freeze thaw cycles.




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