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What is the difference between Km and Kd

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#1 Luria Bertani

Luria Bertani


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Posted 30 May 2011 - 10:52 PM

Hi there,

Just trying to find out what exactly is the difference between the Michealis constant Km and the dissociation constant Kd?

Kind Regards,

#2 castleinthesky



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Posted 22 February 2012 - 06:21 PM

In kinase assay, the Michaelis constant Km is the substrate concentration at which the reaction rate is half of Vmax.
dissociation constant is a specific type of equilibrium constant that measures the propensity of a larger object to separate (dissociate) reversibly into smaller components, as when a complex falls apart into its component molecules, or when a salt splits up into its component ions.

Edited by castleinthesky, 22 February 2012 - 06:27 PM.

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