I am trying to prove protein A binds to my promoter region. Had good sonication of my cell and clear results using anti-histone H3 antibody. I am doing analysis by common PCR. When using my specific antibody (good one from abcam), I see that I have a stronger band using the specific antibody as compared to using rabbit IgG ou no antibody for IP. My PCR gel is attached.
I will be very gratefull if somebody could help me looking at my results and suggesting, because I have no experience.
thanks in advance