Hi people,
I want to dissociate tissue into single cells (primary keratinocytes in fact). Normally, that would be no problem - any trypsin would do. But unfortunately, it is this time crucial for my approach to stay below 20° C. A normal trypsin & co. digestion would take to long even at high concentrations.
Does anyone of you know whether there are some special solutions to this problem? Modified trypsin with an alterated temperatur-activity profile, proteases from Thermophiles, non-enzymatic solutions etc? Do you have any idea whether there are some areas of biosciences where proteolysis at such low temperatures is standardly required? Thank you in advance.
Best regards,
Myschkin
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