Edited by Leon_K, 29 May 2011 - 10:05 PM.
siRNA transfection advise
1 reply to this topic
Posted 29 May 2011 - 09:48 PM
Hi all, i intend to perform a knockdown in U937 and HMC-1 suspension cells using Lipofectamine RNAiMAX. The siRNA oligos are in 20 uM stock solutions and i intend to use 6pmol of siRNA in a 24-well format. I would like to know what volume (micro litres) of siRNA i should add into the media suspension? Any feedback is much appreciated. Cheers.
Posted 30 May 2011 - 07:14 AM
Assume 6 pmol is an amount not a concentration, you need to take 0.3 ul from 20 uM stock to get 6 pmol siRNA.