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isolation of plasmid from yeast

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#1 Penny



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Posted 29 May 2011 - 07:31 PM

Hi. I tried to isolate the transformed plasmid out of yeast by using phenol: chloroform method (details see http://cshprotocols..../1/pdb.prot4150). But the DNA concentration I got is 4700 ng/ul according to Nanodrop results. I was doing this the first time. Is this result normal? Is there any other better isolation method?

And I also have a basic question to ask. After I transfromed the plasmid into the yeast, did the plasmid integrate into yeast chromosome? or it existed as an independent plasmid?

Many thanks,


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