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[margyn87]

Dear all,

Hi, I would like to ask regarding inverse correlation observed in cell culture treatment. One of the factor I use was EGF for differentiation purpose, the more concentrated I put in the media the lesser the number of cells. I wonder whether it's because of toxicity or it induce the cells to differentiate toward terminal and die off. I do believe EGF is a friendly growth factor, so I doubt the cytotoxicity and the fact that I only dilute using PBS plus BSA. Anyone experience using EGF in cell culture that could share opinion?

Thank you so much and I appreciate all the attention.

Best Regards,

Nani