Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

Need help with pre run of PAGE.

  • Please log in to reply
1 reply to this topic

#1 Dr vet

Dr vet


  • Active Members
  • Pip
  • 5 posts

Posted 29 May 2011 - 08:31 AM

Hi there,

I am performing a Urea - PAGE for size separation of products of 112 - 300 bp dna range, Just cant understand the usefulness of pre - run in the whole procedure & how it is done ? Did we have to first load the ONLY loading dye in the wells & then allow the pre run @ say 80 W for an hour OR we just as such pre run the gel to heat it up ? I performed Urea PAGE once with a pre- run , my bands were bowed or V shaped ? does this is because of less time of pre run or the dis-continuation of the pre run that happens due to power failure ?
my general protocol says pre run at 80 W for an hour atleast & then run at 1 & 1/2 hour for size separation .

Pls guide me a little bit for this procedure


#2 mdfenko


    an elder emeritus

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 3,266 posts

Posted 31 May 2011 - 08:41 AM

we pre-run to heat the gel.
talent does what it can
genius does what it must
i do what i get paid to do

Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.