Gentle method to dry a DNA pellet after alcohol precipitation
Posted 29 May 2011 - 01:50 AM
I am looking for a quick but gentle method to dry a DNA pellet after treatment with alcohol (e.g. after Midi preparation or DNA extraction from other tissues).
My approach was to pellet the DNA and then I decanted the supernatant alcohols and put the reaction tube on a liquid soaking paper - upside down.
After a couple of minutes (30 or so) I lay them down horizontally so the alcohol cto allow the alcohol to evaporate.
But this method takes about and hour or so, anyone knows a faster method (except for putting the reaction tube on a 50+°C heating block)?
Posted 29 May 2011 - 01:56 AM
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.
Posted 29 May 2011 - 11:47 AM
I never trust anything that can't be doubted.
Posted 29 May 2011 - 01:12 PM
This sounds very interesting. But either I didn't follow that or you have very special centrifuges that allow you to put a 1.5 reaction tube with the opening down.
Posted 07 June 2011 - 07:44 AM
Wash with 75% Ethanol (1ml) and then after the centrifugation we suck of all the ethanol. P1000 for the majority of ethanol, then a P200 and then the last bits with a P10. If there is drops on the side I either collect them together and suck them off or if they are to little to pipette I smear them on the wall so they become very very small and evaporate easier.
Then I let it sit open and on the side for about 10min. The pellet becomes more white then. So you see that it starts to dry.
Then resuspend, let it sit for 2-3min and then mix.
Do you use GlycoBlue? Makes it waaayy easier as the pellet becomes nicely blue.
Posted 07 June 2011 - 11:30 AM
You need to be somewhat quick about removing the liquid, since as the ethanol evaporates, you are left with water, which will dissolve your pellet. You want to remove the liquid before this happens. In general if you remove all the liquid with a 10 ul pipet tip after centrifuging, you can just leave the tube open on the bench for less than 10 minutes to evaporate the ethanol. You can tell when this is done by smelling it.