yeast glycerol stock
Posted 27 May 2011 - 08:52 PM
Posted 28 May 2011 - 07:40 AM
You can then extract DNA from the yeast and transform the DNA back into competent E coli.
When the expression experiment was repeated, was the growth conditions (culture media, culture size, temperature, shaking speed-oxygenation) changed? It could be that expression of the protein of interest is sensitive to these conditions.
As for re inducing yeast cells, I can not say.
Posted 29 May 2011 - 07:26 AM