I am trying to order some synthetic genes for a protein. The native sequence from the sequence database has a signal peptide and is prokaryotic (i.e. from Bacteria). I have not made any changes to the gene sequence whatsoever.
Should the signal peptide sequences should be removed? Or left?
I am worried about this. Will this express my proteins it in the wrong place in the cell if it has no signal?
Edited by Luria Bertani, 27 May 2011 - 05:09 PM.