Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Is 2% pen strep too much for cell culture?


  • Please log in to reply
3 replies to this topic

#1 Ksu

Ksu

    member

  • Active Members
  • Pip
  • 6 posts
0
Neutral

Posted 27 May 2011 - 11:47 AM

I was making DMEM with 10% FBS and 1% pen-strep, but accidentally made it 2% pen strep. Is this too much? I will be using this on HeLa cells. Will it kill them?
Thanks!

#2 knuf

knuf

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 104 posts
3
Neutral

Posted 27 May 2011 - 11:57 AM

I don't know but I certainly wouldn't chance it. Cell culture media isn't expensive enough to warrant the frustration of dead cells.

#3 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,797 posts
406
Excellent

Posted 27 May 2011 - 04:03 PM

Neither antibiotic will kill eukaryotic cells. However, as kfunk said, chancing something like this and then getting a funny result will (at least, should) result in you thinking to yourself "Is that result because of the change in antibiotic or is it a real result?".. which basically means that you should just make up a fresh lot of medium rather than waste time and money trying to figure out odd results.

The general rule is "If in doubt, throw it out".

Ideally you would not be using any antibiotics in your cell culture. They tend to hide poor technique and cryptic infections which can alter how your cells behave.

#4 philman

philman

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 103 posts
5
Neutral

Posted 31 May 2011 - 02:13 AM

If you're that worried you could just get some more media and combine the two together to double the volume and create a 1% solution (remembering to add extra FCS to keep that at 10%)




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.