hello,i'm Sarah
In order to study interactions between 2 proteins, i'm going to do a dot plot so i will immobilise my first protein on the nitrocellulose membrane then i will do an overlay , i'm looking for a complete protocol of the overlay and informations about this technique. i found articles about overlay in which they do a an electrophoresis and a transfer on the nitrocellulose membrane. so, i can't do this. could some one help me to find a way to do may dot plot and my overlay. i did not find the concentration of proteins that i have to use.
i hope that someone help me.
thank you very much.
0
1 reply to this topic
#2
-
- Active Members
-
- 1,952 posts
an elder
Posted 26 May 2011 - 12:26 PM
dot blots are performed the same way as westerns except that you apply sample directly to the membrane instead of transferring from a gel.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
