hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody
very small insert and a very ling vector
Started by mahrak, May 26 2011 03:55 AM
1 reply to this topic
#1
Posted 26 May 2011 - 03:55 AM
#2
Posted 26 May 2011 - 08:51 AM
mahrak, on 26 May 2011 - 03:55 AM, said:
hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody
What is tough about it? I would suggest digest - purify - ligate. But maybe I am missing a part here... Could you deliver a bit more details?
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