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very small insert and a very ling vector


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#1 mahrak

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Posted 26 May 2011 - 03:55 AM

hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody

#2 Rsm

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Posted 26 May 2011 - 08:51 AM

hey every body
here's my situation: I have to clone a 250bp fragment in a 9400 bp length vector. though one ha?
I was wondering if any one can help me with it. any suggestion or hint is deeply appreciated.
tnx everybody



What is tough about it? I would suggest digest - purify - ligate. But maybe I am missing a part here... Could you deliver a bit more details?
I got soul, but I'm not a soldier




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