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detection of apoptosis


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2 replies to this topic

#1 fadlone

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Posted 25 May 2011 - 06:32 AM

Hi

I'm looking for a way to measure the apoptosis level of human cells inside bioreactors (except LDH level)
Like with ELISA or something else that will give me a value but not microscopy techniques

any ideas?

thanks

#2 noodle

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Posted 02 June 2011 - 06:51 AM

Hi

I'm looking for a way to measure the apoptosis level of human cells inside bioreactors (except LDH level)
Like with ELISA or something else that will give me a value but not microscopy techniques

any ideas?

thanks



I'm not sure if this would be helpful, but have you considered Caspase Activity Assays? We have measured caspase 3 activity level in the past with this method.

#3 Sajjeev Jagannathan

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Posted 17 April 2012 - 05:52 AM

what about annexin V assay using flow?




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