Posted 25 May 2011 - 06:08 AM
I have a question about reference genes. Why when I design assay for some gene the primer sequence are different when I use different reference gene. I thought that primer sequences for my target gene are the same no matter which reference i use.
Posted 25 May 2011 - 10:09 AM
u are comparing different genes ... so ,by default, there will be different primers ...
when I use different reference gene
Posted 25 May 2011 - 10:30 AM