Methods of Methylation detection not requiring bisulfite treatmetn
Posted 24 May 2011 - 10:30 PM
I have very low yield DNA samples that I wish to perform methylation analysis on for a panel of genes. Given their low yield and the loss associated with bisulfite modification, does anybody know of any methylation detection methods not requiring bisulfite conversion? I was considering using a restriction enzyme based method. What have people's experiences been with these methods?
Your assistance is greatly appreciated.
Posted 28 May 2011 - 04:46 PM
Posted 02 June 2011 - 10:06 PM
Thank you for replying to my message. What are the major problems with restriction digestion? I want to validate results obtained using microarray analysis in body fluids of patients with resected cancer to see whether methylation can be detected in low yield samples. I unfortunately do not have access to all equipment required for antibody based methods. What else would you suggest?