ChIP on chondrogenic pellets -- help!
Posted 24 May 2011 - 10:53 AM
I am trying to do ChIP on chondrogenic pellets but cannot get DNA signal on agarose gel at all. Can anybody help me?!! What i did was i ground my pellets on dry ice and passed them through fine needles. At this point the cells should be completely lysed because this is how I extract RNA from this kind of pellets. Then I added nuclear lysis buffer it, sonicated DNA, and ran it on agarose gel. And no signal at all!!!! i am pretty sure the cells should be lysed and I feel it doesn't make sense that I cannot get any DNA signal on the gel. Even the monolayer cells had no DNA signal either!
Can anyone help with this? thanks a million!!!!
Posted 24 May 2011 - 07:53 PM
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