Splice varient quantification in RT real time PCR?
Posted 24 May 2011 - 03:12 AM
Am planning to analyse differential expression of mRNA of my protein of interest by RT real time pcr in treated and untreated cell line.
Problem arised when i checked primers in convential PCR, i found 4 pcr products of different size, sequencing revelaed they are splice varient.
my question: is it advicable to use the same primer which gave 4 pcr product in real time pcr (sybr green based). if not what will be alternative method to quantify my gene of interest.
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Posted 24 May 2011 - 04:38 AM
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