why these guys use T7 instead of CMV?
Posted 23 May 2011 - 09:47 PM
Click here to see the paper
Posted 24 May 2011 - 06:21 PM
In theory you can use any promoter to produce RNA which will then be translated; it all depends on how much protein you want to be produced. If you want lots of protein use a plasmid with CMV or SV40 or other viral promoter as these are typically very high expression as a virus requires lots of protein in a short time. for lower expression you can use mammalian or insect promoters.
Posted 24 May 2011 - 08:55 PM
Bob1, I can't recall how many times you've come to rescue me in the past 4 years, thank you so much.