We start a new methylation specific project. Any suggestion for the best negative control DNA (unmethylated DNA), for the methylation specific pcr? It suggested that we should use placental DNA. Any alternative choice?
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#2
PostDocTrauma
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Posted 26 June 2011 - 12:28 PM
we buy unmethylated dna from milipore.
#3
MoB
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Posted 27 June 2011 - 11:23 PM
Placental DNA is good. You can also try DNA extracted from sperm or whole-genome-amplified DNA (Qiagen-kit).
Edited by MoB, 27 June 2011 - 11:23 PM.
