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negative control for msp pcr


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#1 sofa

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Posted 23 May 2011 - 12:33 PM

We start a new methylation specific project. Any suggestion for the best negative control DNA (unmethylated DNA), for the methylation specific pcr? It suggested that we should use placental DNA. Any alternative choice?

#2 PostDocTrauma

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Posted 26 June 2011 - 12:28 PM

we buy unmethylated dna from milipore.

#3 MoB

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Posted 27 June 2011 - 11:23 PM

Placental DNA is good. You can also try DNA extracted from sperm or whole-genome-amplified DNA (Qiagen-kit).

Edited by MoB, 27 June 2011 - 11:23 PM.





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