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Degradome Deep seq


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#1 Aboozar

Aboozar

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Posted 23 May 2011 - 07:53 AM

Hi all,
I'm going to do a degradome deep sequencing and according to German et al (356 | VOL.4 NO.3| 2009 NATURE PROTOCOLS) we should extract polyA RNA for ligation to the 5' adaptor, Does it need to treat extracted total RNA with DNase before extracting polyA RNA? why?
Thanks




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