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How I have to calculate the theoretical yield of....


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#1 yummianns

yummianns

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Posted 23 May 2011 - 02:04 AM

Hey guys,

I have troubles to calculate the yield of a peptide synthesis. The point is that I dont understand how I have to use the substitution of the aminoacid in the resin. I will put you one example:

Peptide that I want to synthesize: Lys-Ser-Ala-Wang Resin
Fmoc-Ala-Wang Resin (substitution 0.72 mmol/g)

the goal is to synthesize 0.1 mmol peptide so it means that I have to use 0.139 g of the a.a.-resin (m=0.1mmol peptide/0.72 mmol/g = 0.139g).

So how I have to calculate the theoretical yield?

Thanks for advance




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