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Concentrated conditioned medium for western blot - Protein and Proteomics - BioForum

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Concentrated conditioned medium for western blot


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#1 skyyingkai

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Posted 22 May 2011 - 04:03 PM

I ran concentrated conditioned medium on western blot, and I got a huge white colour bands around 60kDa, I think these bands must be from albumin, and my gel ran funny. The conditioned medium containing 10% FCS. May I know how can I get rid of the albumin in the conditioned media after I concentrated them? I want to look at BDNF protein level in the conditioned medium. Thanks!

#2 mdfenko

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Posted 23 May 2011 - 01:08 PM

there are a number of albumin removal kits available.

see this google query.
talent does what it can
genius does what it must
i do what i get paid to do




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