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Isolation of primary myoblasts


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#1 Podge

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Posted 22 May 2011 - 11:37 AM

Hey,

looking for a protocol to isolate myoblasts from my Knockout and WT mice.
Has anybody here worked with primary muscle cells? What's your experience with these cells?

Thank you for you help.

Podge

#2 Podge

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Posted 13 June 2011 - 07:10 AM

In the meantime I have found this great protocol:
My link

#3 DLY

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Posted 16 June 2011 - 07:42 AM

In the meantime I have found this great protocol:
My link



Yup, I got a couple of protocols for that... In my experience myoblasts are best isolated by first isolating satellite cells and then differentiating them into myocytes... Once in culture you will get a quite high level off diffentiation but it will never be completely free from undifferentiated cells and most likely also fibroblasts...




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