Isolation of primary myoblasts
Posted 22 May 2011 - 11:37 AM
looking for a protocol to isolate myoblasts from my Knockout and WT mice.
Has anybody here worked with primary muscle cells? What's your experience with these cells?
Thank you for you help.
Posted 16 June 2011 - 07:42 AM
In the meantime I have found this great protocol:
Yup, I got a couple of protocols for that... In my experience myoblasts are best isolated by first isolating satellite cells and then differentiating them into myocytes... Once in culture you will get a quite high level off diffentiation but it will never be completely free from undifferentiated cells and most likely also fibroblasts...